Why are there low RFU values in qPCR with cDNA compared to large RFU with plasmids for the same mastermix?

I ran an assay with Cy5 probe using Bio-Rad CFX96. Why are some of the curves less intense than some of the other curves? The smaller curves are using cDNA and the larger curves are using plasmid...

01 April 2022 4,636 3 View