Hannah Martin

2 Questions 1 Answers 0 Followers

Questions related from Hannah Martin

Can I degas drug solutions before applying to mammalian cells for electrophysiology?

I am applying different drug solutions to cells that I am whole-cell patching. Each solution is in a syringe connected to a thin tube that goes through a barrel manifold into a glass micropipette...

25 August 2020 2,194 2 View

Why is thrombin failing to cleave its target amino acids in the middle of a 70kDa protein and how do I fix it?

Thrombin is supposed to cleave the correctly sequenced and in-frame LVPR'GS site in the middle of a ~70kDa extracellular protein known to homotetramerize. The goal is to use the N-terminal MBP for...

25 August 2017 6,501 1 View