Hanna Lewandowska

5 Questions 9 Answers 0 Followers

Questions related from Hanna Lewandowska

Why is my copper nanoparticle synthesis not working?

Dear Everyone. I am trying to do the high yield synthesis from ascorbate and copper salt, according to the similar procedures from the literature (0.18M copper ions) I now tried PVA up to 2%,...

22 February 2022 3,551 5 View

Best synthesis procedure for copper nanoparticles in water?

Please can anyone share the best procedure for the synthesis of copper nanoparticles using ascorbate in water? I need a maximum possible concentration and monodisperse size between 10 and 100 nm....

12 February 2022 7,569 5 View

Why can't I see collagen in SDS-PAGE?

I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can...

16 March 2020 10,034 8 View

In what way does Dil (1,1'-dioetadeeyl-3,3,3',3'- tetramethylindocarboeyanine perchlorate) bind to LDL?

I'd like to label my LDL in a manner that would not affect neither thyrosine or cysteine residues of my ApoB100. What does Dil do to the structure of a protein - I can't find it anywhere.

10 September 2014 5,633 3 View

Can anyone help concerning preparative precipitation of LDL?

I haven't got an ultracentrifuge to preparatively isolate LDLs. Is there a possibility to apply the precipitation methods here?

04 October 2013 6,282 0 View