Hanna Kim

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Questions related from Hanna Kim

Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

10 January 2021 4,084 3 View

Western Blot: any reasonable alternatives or troubleshooting advice for punched samples?

Hi, I currently have punched mouse brain tissue separated by brain region (using the cryostat) and the initial plan was to do Western Blot analysis on the punched tissue. We already have the...

02 January 2021 8,995 1 View

Does anyone have experience or suggestions on the polyacrylamide gel method of protein purification and measurement for punched samples?

Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...

02 January 2021 9,561 2 View