Hanif Ullah

4 Questions 9 Answers 0 Followers

Questions related from Hanif Ullah

I need protocol for making cell line immortalized, if anyone have please let me know or at least guide me?

I have sorted cell line but the problem is that it proliferate well upto three or four passages after that their growth slows down making me wait for long time. So i am thinking to make it...

04 April 2019 6,982 6 View

How can i over express the plasmid in THP-1 cells? Which method is the most suitable to get the high efficiency?

Before i did with Lentivirus infection but the cells die, i don't the reason. Also the plasmid size is little long its about 15kb. If anyone have any idea how to get the overexpression of Plasmid...

12 December 2017 7,277 1 View

What is the best method of purification of large protein of more size than 250kDa? if have any protocol please send to my email [email protected]?

Before i use one cell line but expression was very low

11 November 2017 8,304 2 View

How can i get the nice bands of LC3 (human), i tired every method but didn't get any result?

I am working on stem cells and specifically on autophagy

05 May 2016 3,654 5 View