Hanieh Noormohamadi

2 Questions 7 Answers 0 Followers

Questions related from Hanieh Noormohamadi

How are ligation volume and concentration considered?

Hi I have a general query regarding the ligation step in molecular cloning. Does the final ligation volume and concentration, particularly for large inserts and plasmids (3 Kb and 9 Kb), influence...

11 October 2024 7,821 3 View

Could anyone give me advice about my unsuccessful cloning project based on my description?

Hello there I have some difficulties in my cloning project, and I'd appreciate using your experience in this field. I want to clone my insert (2.8 kb, cloned in pUC57) into a manually engineered...

29 September 2024 8,322 13 View