Hania Ramzan

2 Questions 2 Answers 0 Followers

Questions related from Hania Ramzan

Why a primer can produce different PCR product size?

I am using Universal primer set (LCO1490 and HCO2198) to identify Culex mosquito species (tritaeniorhynchus and quinquefasciatus) by using COX gene. the expected product size was 710 bp but after...

07 July 2019 3,667 2 View

Is it possible to get good PCR product if GC content of the primer is lower than 40%?

Is it possible to get good PCR product if GC content of forward & reverse primer is 32% & 35% respectively. Tm of forward and reverse primer is 51.1 & 53.2o C respectively?

12 December 2018 4,979 8 View