Why does my LAMP reaction fail to produce fluorescence, and the amplification is barely visible on 2% agarose gel electrophoresis? Does anyone know?

For the procedure, I prepared the LAMP reagent mix with 1x isothermal buffer, 6 mM MgSO4, 1.4 mM dNTPs, and 9 µL nuclease-free water. I then added a primer mix consisting of FIP and BIP at 1.6 µM...

03 September 2024 7,220 1 View