Hanh Hong Nguyen

3 Questions 15 Answers 0 Followers

Questions related from Hanh Hong Nguyen

Could you share any experience on Sperm DNA fragmentation test using flow cytometry?

Dear Scientists, Could you please share with me any experience about your Sperm DNA fragmentation test using flow cytometry, such as : - Which kit could you recommend? I tried to search some...

12 December 2019 9,126 2 View

How to detect FOXP1 from CD8+T cells by Western blot ?

Can anybody suggest the western protocol for Foxp1 from CD8+ T cell? I used the process but no band can be detected. Total protein 15ug/well. Blocking 4 hours. Primary Ab (Santa Cruz sc- 376650)...

02 February 2015 2,990 6 View

What is the best enzyme mixture for tumor dissociation?

I want to isolate CD8+ T cells from patient tumor and mouse tumor. Nowadays I just use collagenase type I 0.2% and incubate 45 min - 1hour --> Lymphoprep --> MACS isolation. However, my...

02 February 2015 10,005 10 View