Hana Heiba

4 Questions 2 Answers 0 Followers

Questions related from Hana Heiba

Is there a substitute to InhibitEX buffer in Qiagen Stool DNA extraction Kit?

I have a DNA extraction kit and would like to know if there is a way to make my own InhibitEX buffer (or something similar with the same function) to remove PCR inhibitors commonly present in...

24 August 2020 3,382 2 View

What is the best buccal swab sample collection method and preservation for bacterial DNA extraction?

I want to find the best way to preserve buccal swab samples for microbiome studies. I would prefer a dry method (where the swab is not submerged in a lysis buffer or a stabilizing buffer) for ease...

02 November 2019 1,742 3 View

What is the optimum protein concentration to be used for ELISA?

I am using R&D Rat IL-6 kit.

22 May 2017 5,009 3 View

Is there a way to remove/precipitate the fat layer after tissue has been homogenized and protein extracted? The fat affects the BCA results

Rat skin tissue has been homogenized using liquid nitrogen. Lysis buffer is then added along with protease inhibitor. Samples are then used for BCA. Following the BCA the samples will be used for...

22 May 2017 5,867 2 View