Han Xiao

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Questions related from Han Xiao

Does anyone know if transfection of DNA could trigger IFN response in A549 cells?

We used a endotoxin free kit to prepare our plasmids. But we found IFNα proteins could not trigger IFN-dependent reporter genes in A549 cells when compared to mock treatment. We therefore...

10 October 2014 2,155 9 View

Why didn't A549 cells respond to IFNα treatment in my assays?

I transfected A549 cells with IFN signaling reporter plasmids( ISRE/IFNβ/IRF3-firefly luciferase) and CMV-renilla luciferase. 6h later 10,000/1000/100/10 U/ml IFNα2a or IFNα2b proteins were added...

09 September 2014 5,171 2 View