Han Jy

2 Questions 1 Answers 0 Followers

Questions related from Han Jy

Why does my DNA appear no band on the agarose gel?

First, I performed co-transformation using an electroporator device and then picked 15 colonies and cultured them overnight in LB media containing the antibiotic that the recombinant plasmid is...

03 July 2024 9,984 1 View

Does destaining buffer affect the primary antibody signal in the western blot?

After treating my membrane with the primary antibody, the destaining buffer was accidentally used instead of tbst buffer. Immediately after use (about 3 seconds later) it was replaced with tbst...

25 April 2024 2,763 1 View