Hamsa Jameel Banjer

3 Questions 5 Answers 0 Followers

Questions related from Hamsa Jameel Banjer

Many modified genes in cDNA microarray, how to focus on the ones I am interested in?

I have done a cDNA microarray for cancer cells transduced with adeno5 vector holding my gene of interest and the control group was infected as well with adeno5 control ( empty vector) after 24h,...

05 May 2017 9,002 5 View

What is the shortest time needed for a protein to be expressed after Adenoviral vector transduction?

(how quickly the synthesis of first protein molecule is complete) 12h or less or more (not necessary at a detected level but even if it only minimally expressed)

02 February 2017 8,896 5 View

I detected my protein band on western blot at a higher molecular weight (about 30kda increase). What could be the possible explanation?

the protein is a transcription factor subunit of AP1, the sample was treated with B-ME and boiled at 95 degrees for 5 min, ran on SDS-PAGE, could it still be in a dimer after all this?

12 December 2015 6,304 5 View