Hamlet Avilés Arnaut

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Questions related from Hamlet Avilés Arnaut

How many cells will I need to assess the cytotoxicity by the Alamar blue (resazurin) method?

I am working with colon and lung cancer lines HTB-38, HTB-177 and VERO. I put the cells (10,000 or 50, 000 cells/ well with 200 microlitres of DMEM or RPMI+SFB) in a 96-well plate for 24 hr, then...

14 October 2014 9,402 6 View

Can anybody tell me what´s wrong with my cell culture?

I started to see the artefacts inside the red circles one week ago in the culture of human colon cancer cells (ATTC: HTB-38) in DMEM+FBS+Antibiotics (90%+10%+Amp/Strep, 37°C, 5% CO2). The cell...

20 August 2014 9,632 20 View