Hameed Akbar

2 Questions 5 Answers 0 Followers

Questions related from Hameed Akbar

Why eluted protein shows degradation though beads after elution is still clean ?

I tried to purified a GST-tagged protein and I used 0.5% triton-x-100 to wash the enriched beads which resulted in a removal of non specific binding to a great extent. I eluted the protein with...

05 August 2020 3,977 6 View

Purification of his tag protein?

I am trying to purify his tag protein from a Gold strain of E coli, Most of the protein goes to the inclusion bodies at 16 degree induction. I tried 10 degree afterwards and it some how made the...

02 March 2019 6,979 4 View