Hallie Hintz

3 Questions 3 Answers 0 Followers

Questions related from Hallie Hintz

Why did my engineered Ab lose binding affinity when cloned into new scaffold?

I previously developed a mouse single chain variable fragment (scFv) and characterized it after cloning into a human IgG1 scaffold. Had great potency and selectivity in vitro and in vivo. I now...

03 July 2019 3,341 4 View

Buffer for transmembrane protein extraction from mouse tumor tissue?

I will be using the protein for ELISA and want to make sure that the transmembrane protein of interest is not denatured or degraded.

02 August 2018 1,260 4 View

Recommended protocol for MDSC differentiation from PBMC?

Can someone recommend a protocol for differentiating MDSC from donor PBMC?

29 January 2018 1,086 1 View