Can anyone help me with problem where 75uM Hoechst 33342 in a A-375 cell culture tends to precipitate?

I am studying the anti-tumor effect of Hoechst33342 dye on A-375 cells. However, when I added 75uM of the dye to the plated cells, the dye seem to precipitate and I get close to 100% cell death....

17 July 2014 6,245 3 View