Hale Tasyurek

3 Questions 5 Answers 0 Followers

Questions related from Hale Tasyurek

Does anyone have any suggestions for harvesting lentiviral vectors produced by CaPO4 transfection in the presence of chloroquine?

We are changing some parameters for producing high tiger lentiviruses. So we use Chloroquine as a transfection agent at the concentration 25uM for 8 hours. Than changing the media. So far we...

05 May 2014 5,587 4 View

Is it necessary to use heat inactivated FBS for MIN6 cell culture?

We are culturing MIN6 cells in our lab. And we use complete medium with %10 FBS, which is heat inactivated according to protocols. But I would like to try to use non-heat inactivated FBS. Is it...

02 February 2014 5,918 2 View

Lentiviral vector resuspension and parameters of ultracentrifugation step?

We are producing Lentiviral Vectors with CaPO4 transfection. And trying to optimize our protocol. There are two steps that we couldn't decide. One of them is the speed and degree of...

02 February 2014 9,548 5 View