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Using western blot, LC3-II ( rerpresenting the autophagosomes or the autolysosomes) decreased in treated cells compared to control while using IF, LC3 puncta ( rerpresenting the autophagosomes or...
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Hello, I am treating my cells with cloroquine to block autophagy at the late stage. Using Western blot, I found that the LC3 II form is accumulating; however, the P62 protein is not changing (it...
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