Hala Shallhoub

3 Questions 10 Answers 0 Followers

Questions related from Hala Shallhoub

I had got contradictory results concerning the LC3 protein between western blot and Immunofluorescence. Any explanations?lanation?

Using western blot, LC3-II ( rerpresenting the autophagosomes or the autolysosomes) decreased in treated cells compared to control while using IF, LC3 puncta ( rerpresenting the autophagosomes or...

18 September 2020 6,665 3 View

Question related to autophagy and autophagy related proteins?

Hello, I am treating my cells with cloroquine to block autophagy at the late stage. Using Western blot, I found that the LC3 II form is accumulating; however, the P62 protein is not changing (it...

02 November 2019 1,116 7 View

Autophagy depends on the fusion between autophagosomes and lysosomes.Are autophagy induction and lysosomes bio-genesis induction mutually inclusive ?

Is lysosomal number or its location ( peri-nuclear vs spread through the cytoplasm) which indicates its induction?

01 January 1970 1,425 1 View