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I'm cloning 1047 bp insert in 9508 bp vector using BstxI and NotI. Transformation seems to be efficient every time. However, when performing the diagnostic restriction digest of grown colonies;...
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I am dealing with a predicted 40 kDa MAP kinase in WB. I don't have access to LC/MS to make sure of its MW, so I rely on that of the strongest band detected (I'm not worried about faint ones...
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