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Questions related from Hajar Alghamdi
what is the recomended conc. to prepar cDNA to run rt-pcr for brain rat tissue.. i used 50ng/ul some primer gave ct value btw 20-22 but other above 30.. ?
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i did RNA extraction using differnt protocol triazol and invetrogen RNA mini kit .. the 260/280 ratio was 1.9 to 2.1 but for the 260/230 i got 1.5 to 1.8 in both protocols, should i consider it...
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