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Questions related from Haitao Wang
I have a dataset, assoicated with 5 time points. For each time point, I have three replicates. I understand that for a time-series dataset, repeated-measures ANOVA or Friedman test would be the...
10 January 2022 5,529 7 View
I have hundreds of samples with different sample sizes/counts (3,000 to 150,000). Due to the uneven sizes, comparing the richness between samples can be tricky without rarefying. However, if I...
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I have created co-occurrence network based on Spearman's correlations for several times with different projects. My research focuses on microbiome. So the networks were the correlations between...
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I'm quite confused about using DESeq2 to find the differential abundant taxa in microbiome studies, especially when there are more than two groups of the factor. I know DESeq2 was initially used...
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I'm wondering if there is a clear method that can be used to create the bipartite network to connect the samples with OTUs/taxa. As I searched the internet, all of the tutorials are talking about...
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I used adonis to test the difference between groups/categories based on the 16s high-throughput sequencing data. I got a very significant result (p = 0.001), but the R2 is very low (< 0.1). I...
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I want to change the current microbial communities in one soil into the communities from the other soils. I'll first sterilize the former soil. So question is how can I inoculate sterilized soil...
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