Does a 3 amino acid insertion (Alanine) between the stop codon of tag protein and 3' UTR of endogenous protein affect fusion protein structure?

Hello, I am currently working on making a fusion protein through the tagging of an endogenous gene. I have restricted and ligated fragments such there has appeared a 3 amino acid (Alanine...

04 July 2024 8,219 1 View