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Questions related from Haemin Lee
Purpose : Transcription factor expression in cell(Raw264.7) Method : 1.(ver.1) pCIneo constitutive expression vector + target cDNA are inserted, (ver2.Flag tag added to 3`end of target cDNA), my...
09 January 2020 5,197 0 View
Below protocol is "A-beginners-guide-to-ChIP" from Abcam 3. Determination of DNA conc part (after "1.Cross-linking ~ 2.Sonication" ) Summarized protocol is : 1.11 : PCR purification 1.12 : RNase...
31 May 2019 3,324 2 View
I recently used pSpCas9(BB)-2A-Puro(PX462) for knock out the targets in Raw264.7 cells. During experiment from cloning sgRNA to puromycin selection, i thought that EGFP-selection method is better...
16 April 2019 7,767 2 View
in my SDS PAGE gel Top and middle is good but Bottom part always have smear band Size of my target protein is 17kDa on the photoshop, i edit the picture, but also it can not cofirm.
09 January 2019 3,568 4 View