Haemin Lee

4 Questions 4 Answers 0 Followers

Questions related from Haemin Lee

What should be taken consideration to solve the problem of western blot not detected after transfection with pCIneo plasmid containing target cDNA?

Purpose : Transcription factor expression in cell(Raw264.7) Method : 1.(ver.1) pCIneo constitutive expression vector + target cDNA are inserted, (ver2.Flag tag added to 3`end of target cDNA), my...

09 January 2020 5,207 0 View

ChIp : Determination of DNA concentration from Abcam?

Below protocol is "A-beginners-guide-to-ChIP" from Abcam 3. Determination of DNA conc part (after "1.Cross-linking ~ 2.Sonication" ) Summarized protocol is : 1.11 : PCR purification 1.12 : RNase...

31 May 2019 3,327 2 View

Could anyone recommend a CRISPR/Cas9 plasmid(Facs sorting, EGFP)?

I recently used pSpCas9(BB)-2A-Puro(PX462) for knock out the targets in Raw264.7 cells. During experiment from cloning sgRNA to puromycin selection, i thought that EGFP-selection method is better...

16 April 2019 7,774 2 View

What is the problem of smear on the bottom of SDS PAGE gel?

in my SDS PAGE gel Top and middle is good but Bottom part always have smear band Size of my target protein is 17kDa on the photoshop, i edit the picture, but also it can not cofirm.

09 January 2019 3,575 4 View