Hadrien Peyret

2 Questions 8 Answers 0 Followers

Questions related from Hadrien Peyret

Can one make homemade 2X reaction buffer for the SuperScript III one-step RT-PCR kit?

I do a lot of RT-PCR and always include a no-RT control (without reverse transcriptase) for each of my samples, so that I can rule out DNA contamination in my RNA samples. Unfortunately, the...

06 June 2016 4,242 9 View

How do I optimise PEG precipitation?

I am trying to purify a heterologously-produced virus-like particle, and PEG precipitation (or ammonium sulfate precipitation) would really help. I have done a couple of trials but they didn't...

04 April 2013 4,764 26 View