How to revive hybridoma cells adapted to serum free media after cryopreservation ?

I have been growing my hybridoma cells inGibco Hybridoma SFM . I have cryopresrved them using 92.5% medium (50:50 ratio of fresh to conditioned media) +7.5% DMSO , the viability was 87%. i froze...

10 October 2017 710 1 View