Avoiding inclusion bodies formation during IPTG induction?

I have tested several induction conditions for expressing my protein, I am using the pet28b vector, expressing in E.coli BL21(DE3). First I started with 0.1mM/ 0.2mM /0.5mM/ 1mM IPTG at 37C for 5...

14 May 2013 2,829 22 View