H Surachandra Singha

4 Questions 6 Answers 0 Followers

Questions related from H Surachandra Singha

What is the optimum conc. for miRNA cDNA to perform qPCR experiment?

Hello, I have prepared miRNA cDNA from 50ng RNA and based on the kit (Qiagen miRCURY LNA miRNA Assay) it is suggested i perform 60X dilution of the cDNA whish would bring down my RNA conc. to...

04 April 2019 8,772 4 View

What approach would be good for micro RNA expression analysis?

I want to analyse the expression of miRNA through RT-qPCR method. I am confused with the various approaches of doing it. Should I go for designing the primer (Stem loop primer) to synthesize the...

08 August 2018 8,367 3 View

Is Real Time PCR annealing temp. different from normal PCR temp.?

My RT primers have suggested annealing temperature of 42 degree celsius. I tried normal PCR with this annealing temperature and it showed considerable bands. Now, while running it on Real Time PCR...

02 February 2018 4,758 7 View

How to collect hypothalamus?

I am looking for a good procedure to collect the hypothalamus from an avian brain without disturbing the form of the organ.

10 October 2017 9,058 1 View