Hakeem Ayomi Sanni

3 Questions 8 Answers 0 Followers

Questions related from Hakeem Ayomi Sanni

Please how long does the staining stay on cells, can you still image after 24hrs and the stained cells will still retain their stain?

The idea of fixing the cells with 4% Paraformaldehyde/ ethanol is to make sure the cells do not wash off and are still present during the whole process .So if I wanted to do this experiment the...

02 February 2016 8,156 4 View

I'm trying to stain cancer cells grown on gel. I'm not sure whether I need to fix my cells with 4%PFA or ethanol before staining?

I am trying to stain #ovarian cancer cells grown on gel with ethidium bromide and calcien. I am not too sure whether to fix my cells with 4% PFA or ethanol before staining. Any suggestions will be...

02 February 2016 5,349 5 View

Staining cells growing on gel with ethidium bromide and calcien. This is an addendum to my earlier question?

The idea of fixing the cells with 4% Paraformaldehyde/ ethanol is to make sure the cells do not wash off and are still present during the whole process .So if I wanted to do this experiment the...

02 February 2016 1,230 3 View