Gururaj Kumaresan

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Questions related from Gururaj Kumaresan

How to avoid background signal in dot ELISA?

I am standardizing indirect dot ELISA for detecting anti-epsilon antibodies in Enterotoxaemia vaccinated goats. I have coated the combs with toxin antigen, where titrations of...

12 December 2017 5,186 11 View

In sandwich ELISA, whether the coating antibody and primary antibody should be raised from different species or should belong to the same species?

Suppose the coating antibody (may be against some pathogen affecting chicken, for instance Newcastle disease virus i.e anti-NDV IgG from chicken) used in the sandwich ELISA is raised in chickens,...

12 December 2017 1,193 4 View