Gurcharan Kaur

4 Questions 6 Answers 0 Followers

Questions related from Gurcharan Kaur

What could be the reason for no amplification in SYBR green QPCR while getting amplification in TaqMan QPCR?

I am trying to run QPCR on NK cell RNA using SYBR green. I have not been getting any amplification even in the 18S gene which we use as control. While the same primers and SYBR green works well on...

24 September 2018 1,351 3 View

What could be the reason of detachment of immobilized suspension cells in seahorse experiment?

I am doing SeaHorse experiments (mito stress test) for NK cells. I use cell tak to immobilize the cells to the cell plate. I follow all the instructions as provided in Agilent protocol for...

14 August 2018 4,832 1 View

Which method between Cell-tak and Poly-D-lysine coating is better for suspension cell immobilization for seahorse assay?

I am planning to do seahorse analysis of suspension cells. I have come across protocols using both Cell-tak and Poly-D-lysine to immobilize suspension cells to the well for the assay. I wanted to...

10 August 2018 9,000 9 View

Can anyone tell a good method to isolate RNA from NK-92MI cells?

I have been experiencing difficulties in isolating mRNA from NK-92MI cells. I am using Direct-zol RNA isolation kit right now to isolate RNA form 5 million cells per sample. After making cDNA,...

02 July 2018 5,187 9 View