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Hello everyone. I'm trying to make CRISPR knock-in cell line. Maternal cell line is HCT116 which is known as diploid cancer cell. I want to knock-in fluorescence protein into C-term of my target...
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Hi. I'm trying to produce lentivirus. However, I had found that there are several kinds of lentiviral transfer vectors containing LTR pair on both hands of GOI coding region. 1) 5'LTR and...
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Hi, I'd like to perform transduction HeLa cell with Lenti-virus. According to general protocol, it says spin infection step is needed after virus containing medium treatment. I prepared lentivirus...
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Hi, I'd like to make stable cell line expressing particular gene. And I have the plasmid containing that gene. This plasmid is retro viral vector. But I want to deliver these plasmids into cell by...
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