Gulnaz Begum

3 Questions 8 Answers 0 Followers

Questions related from Gulnaz Begum

What is the best cell isolation method to study the BBB transcriptome by single cell RNAseq analysis?

I am trying to do the single cell RNAseq of BBB specific cells, importantly the endothelial and mural cells from mouse stroke brain samples. I am finding it hard to decide whether I should isolate...

30 March 2023 6,722 4 View

Setting a threshold for analyzing data?

I want to know if I have to keep the threshold same during analysis to compare two sets of data? for example I have a control image, when I threshold it the minimum is 30 and maximum is 255, for a...

06 February 2019 2,691 2 View

Co-labeling with Brdu and nuclear stain To-Pro3?

Hi, I am trying to co-label tissue sections with Brdu and To-pro-3. Interestingly when I immunostain for Brdu and Neun/GFAP I can see very nice staining but when I include Topro-3 no Brdu positive...

07 December 2015 4,798 7 View