Guillaume Grzych

3 Questions 1 Answers 0 Followers

Questions related from Guillaume Grzych

Why do we get smear after PCR on agarose electrophoresis?

We getting smear on our gel agarose using SYBR Green I Nucleic Gel Stain (1X diluted from 10 000X solution) in 2% gel agarose. We got smear on our 100 pb ladder (on left and right on picture) and...

12 December 2016 3,283 6 View

Does anyone have experience transfecting SH-SY5Y cells with Effectene?

The plasmid I transfected into is about 10Kb.

11 November 2016 9,625 1 View

Is it possible to generate stable isolated clones of SH-SY5Y Ko cells line (with CRISPR/Cas9) using FACS (mCherry) ?

We want to make stable SH-Sy5y Cell line Ko cell line after Transfection with CRISPR/Cas9. We looking the best way to isolate our transfected clones (FACS or Puromycin).

11 November 2016 4,521 1 View