Late refinement, phenix.refine marginally improves geometry while Free residuals get much worse. What could be a better refinement strategy?

I am refining the crystal structure of a protein that is around 20 kDa. I am getting towards the point where most of the validation statistics and residual look nice: resolution 1.85Å, Rfree=19.5%...

24 February 2020 4,244 1 View