Guillaume Bompard

3 Questions 9 Answers 0 Followers

Questions related from Guillaume Bompard

Cloning issue: mixed colonies how to get rid off them?

Hi, I'm facing a recurent problem with the cloning of a given cDNA. Everytime I'm doing the cloning I end up with a mix of big and small colonies on the insert+vector plate while getting only few...

04 January 2024 9,912 3 View

Baculovirus production issues, how can I improve things?

Hi all, Once again I'm facing some issue regarding baculovirus production. I'm using the Bac-to-Bac system and got nice protein expressions after bacmid transfection (immunoblot and visible by...

24 June 2016 8,027 10 View

Issue with His-tagged protein elution, how can I solve it?

Hi everybody, I'm facing a problem with the purification of an His-tagged protein produced in Sf9 cells. Protein, which is also GFP-tagged, is nicely expressed (unlikely cytosolic), easily...

17 March 2016 8,125 4 View