Guilherme Souza

6 Questions 7 Answers 0 Followers

Questions related from Guilherme Souza

Tukey or Multiple t-tests ?

Hello everyone. I am conducting an experiment to evaluate if a compound inhibits a parasite growth quickly or slowly. To do this, I measure the IC50 of this compound when it acts for 24h, 48h or...

08 September 2020 5,556 5 View

Confidence Interval of IC50 ?

Hello everyone. I work with drug development, and routinely conduct IC50 measurements. Usually I report IC50 as sample mean +- sample standard deviation. Now I have been looking on the correct way...

07 September 2020 1,466 4 View

Sample and Population of IC50 values ?

Hello everyone. I work with drug development, and I have been trying to apply some basic statistics concepts in my analysis. I would like to understand better what is a sample and what is the...

07 September 2020 3,497 3 View

Can't see inhibition happening in assay

I'm working with a coupled assay involving my main enzyme, Enolase, e two subsequent enzymes: pyruvate kinase, which transfer the enolase's product phosphate to an ADP molecule, producing ATP, and...

05 July 2017 7,919 0 View

Help with coupled enzymatic assay?

I am trying to re-determine the Km of the enzyme I'm working with. For some reasons, I'm trying to do this using a coupled assay. I am having trouble with that, because the Km that I am finding is...

27 January 2017 6,828 2 View

In size exclusion chromatography, why is column volume calculated by the volume of the cylinder holding the resin? What about the beads volume?

I'm asking this because I'm using a 500mL column, which volume is calculated by the cylinder volume, and I can see (conductivity using AKTA) that its internal volume is indeed 500mL. Shouldn't it...

18 January 2017 5,886 8 View