Guilherme Crispim de Faria Cruz

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Questions related from Guilherme Crispim de Faria Cruz

Have anyone tried to separate imidazole from His-tag recombinant protein using FPLC?

Hi there! I am studying protein ligand interactions by STD-NMR and the problem is that the imidazole, used in the Ni-NTA puriffication step interferes a lot in my NMR experiment. I even dialysed...

27 February 2019 4,724 8 View

Is there an oxidative alternative for DTT in sample preparation buffer for SDS-PAGE loading?

Hi there! I'm purifying a recombinant protein and I always get 2 spots in SDS-PAGE in which one spot has two times more mass than the other. Probably it's a dimer. I would like to try an...

12 December 2018 3,104 4 View