Guglielmo Bove

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Questions related from Guglielmo Bove

I'm doing RNA Dot blot assay for m6A detection but I'm struggling with reproducibility. Any tips to improve manual RNA spotting on membrane?

We also have a Bio-dot apparatus available now, but the RNA seems not to stick to the membrane. I would be glad if you could share suggestions/experimental protocol for that

06 December 2021 765 5 View

Why do I get weird histone bands with my Hep G2 cells??

I'm performing standard histone extraction (abcam protocol) and western blot essay on my HepG2 cells but the band's that I get don't seem to have the classic histone shape. Any clue guys?

15 December 2020 1,859 3 View