Gudani Tshiswaise

2 Questions 3 Answers 0 Followers

Questions related from Gudani Tshiswaise

How to conduct folded fraction from intrinsic tryptophan fluorescence spectroscopy?

I am conducting a study on DnaK with four compounds that are known inhibitors of human Hsp70. As part of this, I performed thermal denaturation assays at 20°C, 40°C, 60°C, 80°C, and 100°C to...

04 November 2024 8,579 1 View

Is it a must for Bradford assay trendline to intercept at zero, or does it depend on whether the equation should be 𝑦 = mx 𝑥 or 𝑦 = mx + c?

I have been struggling to determine the protein concentration, which is crucial for optimizing the working concentration needed for my research assays. Unfortunately, I have been receiving...

30 May 2024 2,335 4 View