Guannan Wang

2 Questions 7 Answers 0 Followers

Questions related from Guannan Wang

Should I expect to see a distinct band after CsCl gradient purification of AAV8 virus?

Some suggest measuring refractive index, some suggest collecting all fractions and selecting virus-rich fraction by qPCR. I'm wondering why after purification I do not see the formation of a...

23 July 2014 2,714 12 View

Is one expecting to see GFP expression in AAV-293 cells transfected with liver-specific promoter TBG AAV packaging plasmids?

We are trying to purify GFP-AAV8 virus from AAV-293 cells, and the GFP is under the TBG promoter in the trans-plasmid. After transfection, green cells are observed under the scope. My questions...

18 July 2014 3,288 7 View