Guan-Yi Li

4 Questions 1 Answers 0 Followers

Questions related from Guan-Yi Li

How to compare two X-ray protein structures by RMSD, focusing on α-helices?

Hi everyone, I am currently working on merging protein structures obtained from multiple X-ray diffraction datasets. After refinement, I noticed that there are some structural differences between...

last month 1,916 1 View

How do i use different XRD data from different crystal but all of them are from same protein even same conditions?

I use HKL-2000 to process my XRD data. One of them has a resolution of 2.3 Å, but I also have other XRD datasets with resolutions worse than 2.5 Å. Can I use these different datasets to improve...

24 August 2023 9,740 1 View

How should I select refinement strategies when attempting to reduce the R value after employing molecular replacement?

I frequently utilize Phenix.phaser and Phenix.refine, along with CCP4i, to address these issues. Despite numerous attempts through trial and error, I have not yet formulated a definitive protocol...

09 August 2023 5,484 2 View

What the meaning of the electron density maps?

Dear community. I'm just studying for a master’s degree in the department of biochemical science & technology, at National Taiwan University. When I try to refine the structure at Coot or...

18 November 2022 562 3 View