Griffin A. Beach

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Questions related from Griffin A. Beach

Using a detergent free blocking solution for immunohistochemistry, would I simply just omit the detergent or is there a suitable substitute?

I am screening antibodies and I discovered that one of them is listed on DSHB (Developmental Studies Hybridoma Bank) as being detergent sensitive. I am wondering what would vary from my normal...

08 August 2019 7,647 3 View

Any recommendations on how to process the pellet from homogenized snail tissue to extract membrane proteins for western blot analysis?

I have been homogenizing snail tissue with acetic acid, and then using ammonium sulfate precipitation to extract proteins from the retrieved supernatant. However, I am worried that the target...

04 April 2019 7,898 8 View