Can 0.1M Ba(OH)2 precipitate 500 ug of peptides (HEK cell line)?

I was doing beta elimination with 0.1M of Ba(OH)2 at 37 and 55 degrees for 2 and 24 h. In all conditions I lost more than half of the peptides. How can this be explained?

18 June 2019 1,386 2 View