Graciana Willis

5 Questions 10 Answers 0 Followers

Questions related from Graciana Willis

Any expirience in concentrate PCR products form 600 bp by liofilization?

I have many samples of a PCR product from 600 bp to concentrate and purify, the kit is not worth and I don not have a speed vac centrifuge. The PCR product is for DGGE application, using archaea...

10 October 2017 2,964 3 View

Does anyone know the composition of E.Z.N.A Soil DNA kit solutions?

We are having problems with this kit. Low DNA yield, as we obtain 5 ng/ul of DNA from a sediment sample and biofilms. Samples are quite different so we think that there is some problems with the...

03 March 2015 8,347 7 View

How can I make a concentrated ferric iron solution at pH 4.0?

We are having some problems on preparing a concentrated ferric iron solution ( ppm) at pH 4.0. Dissolving ferric iron at low pH is easy but when we increased the pH most of the iron is...

03 March 2015 7,379 4 View

Which is the best way to extract DNA from a culture of gram-positive bacteria?

I am trying to do DNA extraction from two strains of gram-positive sulfate reducing bacteria which form MeS precipitates (Zn and Fe). I am having some problems with DNA yields, they are two low!...

03 March 2015 7,886 6 View

What should I do during DNA extraction with phenol:clorophorm:isoamilyc, to get high molecular weight DNA?

I am doing DNA extraction for some gram-positive sulfate reducing bacteria. I treated the pellet with lysozyme overnight and then added RNase for 1 hr at 37 °C. then I used proteinase K, and...

03 March 2015 2,347 12 View