Grace Young

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Questions related from Grace Young

If bacterial cells are completely lysed following treatment with an antimicrobial- then washed- then PI stained- Why is concentration lower?

Hi- I have analysed some PI stained samples through flow cytometry. The results show a much lower concentration of cells in those samples which have been treated than those which haven't...

25 July 2023 7,419 2 View

Zetasizer Nano ZS- water filtered through 0.2um but still showing presence of "large or sedimenting particles" ?

Hello, I am having issues to the Zetasizer Nano ZS that I was hoping someone could give me some ideas to try and fix please. I am attempting to run critical micelle concentration analysis with the...

14 May 2021 9,141 4 View

Has anyone struggled with growth from staph aureus strain in CAMHB when diluted to 10^5CFU/ml?

I am trying to grow S.aureus NCTC 6571 in Cation-adjusted Mueller Hinton Broth. I need this to be at a starting inoculum of 10^5CFU/ml for antimicrobial susceptibility testing. I am diluting...

02 November 2020 3,779 4 View