Gowri Kanagasabapathy

2 Questions 2 Answers 0 Followers

Questions related from Gowri Kanagasabapathy

What is the best kit for protein extraction?

I'm going to do Western Blot and I would like to know what is the best kit to use to extract the protein from adipocytes cell (3T3-L1)?

05 May 2013 1,512 2 View

Do I have to run 2 housekeeping genes (18S and beta-actin) together in each run in a Real-time PCR?

I'm running Real-time PCR on STEPONEplus instrument (singleplex) which allows me to choose only one endogenous gene. However, can I run another endogenous gene in the same 96 well? I would also...

05 May 2013 2,432 2 View