Gowri Shankar

2 Questions 3 Answers 0 Followers

Questions related from Gowri Shankar

Need help for Site directed mutagenesis using Phusion...

Hi all, I trying to do single site directed mutagenesis with my 6.4kb plasmid. Initially I designed the primers around 40nt long, but since both the primers are exactly complementary, I was...

21 March 2016 854 9 View

I want to strip a western blot. Can you suggest an efficient method?

I have stripped with a harsh stripping reagent such as B-mercapto ethanol but I lost most of the protein

25 April 2012 705 18 View