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I am working with neuro2a cell line.I grow them in DMEM with 4.5g/L glucose medium. For the last few months, cells are forming debris and have started looking stressed. This problem persists even...
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I am coating the plates with Poly-L lysine for better attachment of differentiated neuro2a cells. I want to know whether it can come in the media and affect mass spectrometric analysis of secretome.
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