Gopalakrishnan Chandrasekaran

4 Questions 5 Answers 0 Followers

Questions related from Gopalakrishnan Chandrasekaran

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

08 June 2020 2,281 2 View

What is the best method for homogenising Testis tissue for RNA isolation?

I want to isolate RNA from testicular tissue using Trizol. Everytime when I run the gel after isolating RNA, the bands are getting smeared. All the precautions have been taken care. All the...

20 November 2015 3,387 5 View

Which is the best online tool for miRNA 3'UTR SNP prediction and network analysis?

For Splice site mutations, miRNA seed sequence, cytoscape, etc.

09 March 2013 6,139 2 View

Are there any possible micro RNA's related with schizoprenia? Are any of them also reported in Cataracts?

miRNA related SNP's

08 March 2013 5,799 2 View